Translational_Unit

Part:BBa_K2149016:Design

Designed by: André Tomas Vilela Hermann   Group: iGEM16_USP-EEL-Brazil   (2016-10-13)


luxE (Acyl-protein synthetase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 988
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 927
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 730
    Illegal AgeI site found at 945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 565


Design Notes

The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap. Its design takes into consideration a RBS and a coding sequence.


Source

This part was synthesized by IDT, and it comes from the genomic sequence of Photorhabdus luminescens, codon optimized for Escherichia coli. The sequence was taken from the accession number JQ901710, which was used it to write the article "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli".

References